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Anti-PI3K抗體現(xiàn)貨供應(yīng)

時(shí)間:2014/4/2閱讀:290
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  Anti-PI3K抗體現(xiàn)貨供應(yīng)

FROM: ABCAM

Catalog Number : BYK-0128R       
Quantity size :   100ug  (0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide)

Anti-PI3K抗體現(xiàn)貨供應(yīng)Background: The enzyme phosphatidylinositol 3 kinase (PI3 kinase) is a lipid kinase that generates phosphatidylinositol 3, 4, 5-triphosphate in response to receptor activation in many signal transduction pathways. Class IA PI3Ks exist as a heterodimer of a catalytic 110 kDa (p110) and a regulatory p85 subunit (e.g. p85 alpha). p85 alpha is an adaptor molecule that regulates the activity of the catalytic p110 subunit by binding to phosphorylated receptor tyrosine kinases (RTKs) through its SH2 domain and mediating the interaction between p110 and the plasma membrane. p85 alpha is necessary for insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.

Specificity :   

  • Anti- PI3K is a mouse monoclonal antibody unconjugated                
  • specific for PI3K of Human, Rat, Chicken, Dog, Cow, Horse          
  • use for western blotting,elisa,immunoprecipitation and immunohistochemistry
  • Protein G affinity chromatography purification, purity :>95%
  • Isotype:  IgG
  • molwt:  80kDa

Anti-PI3K抗體現(xiàn)貨供應(yīng)Application :  

  • Western blotting 1:100-500                
  • Immunohistochemistry 1:100-500                  
  • ELISA 1:500-1000
  • IP=1:20-100 
  • IF=1:100-500                  
  • Optimal working dilutions must  be determined by the end user.

Storage:  Store at -20 for one year. Avoid repeated freeze/thaw cycles. The  lyophilized antibody is stable at room temperature for at least one month and for greater  than a year when kept at -20. When reconstituted in sterile pH 7.4 0.01M PBS or diluent  of antibody, the antibody is stable for at least six weeks at 2-4

Anti-PI3K抗體現(xiàn)貨供應(yīng)Important Note: This product as supplied is intended for research use only, not for use in  human, therapeutic or diagnostic applications.

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